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To generate the GFP-fusion of Go-Nt 7 and Go-Nt 31 , the fragments were PCR-amplified (primers listed in Supplementary Data 3) from Go-GFP, products were cut with KpnI/AgeI and cloned in frame into the same sites of pEGFP-N1 (Clontech)

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Possible warning symptoms: Fatigue Skin dullness, hyperpigmentation, and other signs of premature skin aging (loss of skin elasticity, roughness, and wrinkling) Foggy brain Poor sleep Aches and pains Frequent infections Symptoms of Severe Deficiency: Anemia (low levels of iron) Metabolic acidosis (build-up of acids in the body) Liver disease Loss of coordination (ataxia) Seizures and neurodegenerative diseases (e.g., Alzheimers, Parkinsons) Heart attack and stroke Natural Ways to Promote More Glutathione No surprises

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